PCR & Primer Design Tools
Oligonucleotide duplex annealing parameters, reaction scaling, and master mix setup wizard.
Primer Pair Evaluator
Estimate oligonucleotide melting temperatures ($T_m$) using nearest-neighbour approximations suitable for standard Taq reaction buffers.
Forward Metrics
-|-|0 bp
Reverse Metrics
-|-|0 bp
PCR Master Mix Scaler
Includes standard +10% pipetting overage factor to account for meniscus loss during multi-tube dispensing.
| Reagent Component | Per Reaction (µL) | Master Mix (µL) |
|---|---|---|
| PCR Buffer (10x) | 22.00 | |
| dNTPs (10 mM) | 5.50 | |
| Forward Primer (10 µM) | 5.50 | |
| Reverse Primer (10 µM) | 5.50 | |
| Taq Polymerase | 2.20 | |
| Nuclease-Free Water | 168.30 | |
| Total Volume | 19.00 µL + 1.0 µL DNA Template | 209.00 µL |
Disclaimer: This toolkit is intended to support trained laboratory professionals. It provides calculation and decision-support functions and should be used in conjunction with local SOPs, validated laboratory procedures and current national guidelines. Final clinical interpretation remains the responsibility of the reporting laboratory.