Virology & Cell Culture Tools
Multiplicity of infection (MOI), viral titre volumetric dosing, and inoculation calculators.
Calculate Required Inoculum Volume
Determine the exact volume of virus stock needed to achieve your target MOI based on your cell count and viral stock titre.
Formula: Volume (mL) = (Target MOI × Total Cells) / Virus Titre (PFU/mL)
Calculate Resulting Actual MOI
Calculate the actual multiplicity of infection administered given a specific volume of viral inoculum added to culture.
Formula: Actual MOI = (Volume (mL) × Virus Titre (PFU/mL)) / Total Cells
Disclaimer: This toolkit is intended to support trained laboratory professionals. It provides calculation and decision-support functions and should be used in conjunction with local SOPs, validated laboratory procedures and current national guidelines. Final clinical interpretation remains the responsibility of the reporting laboratory.